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Suggest a method you could use to estimate the concentration of glucose in several different solutions that all turned brick red with Benedict's reagen in 3 minutes.

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Suggest a method you could use to estimate the concentration of glucose in several
different solutions that all turned brick red with Benedict's reagen in 3 minutes.

Suggest a method you could use to estimate the concentration of glucose in several different solutions that all turned brick red with Benedict's reagen in 3 minutes.

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EllisVeteran · Tutor for 9 years

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By comparing the color of Benedicts test to a "standard chart" or by using a colorimeter and exploiting a created standard curve (absorbance vs. concentration), we can effectively determine an estimate for the glucose concentration for multiple solutions having turned brick red after being exposed to Benedict's reagents after 3 minutes.

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## Step1: <br />Before we can suggest a method, we really need to understand what happens when solutions turn brick red with Benedict's reagent. Benedict's Reagent is used to test for reducing sugars. When a reducing sugar is mixed with Benedict's reagent and heated, it changes color. The color varies from blue (no reducing sugar...as Benedict's solution is naturally blue), green (trace), yellow (small amounts), orange (moderate amounts) to red (high amounts). Since we are discussing solutions that all turned brick red, this would inform us that there are high levels of reducing sugar present, which in this case is glucose. <br /><br />## Step2:<br />Following, knowing that color change indicates the presence and concentration of glucose, we can establish a method using standard solutions, exploiting this very property.<br /><br />### Preparation of Known Concentration of Glucose Solution:<br />First, a series of glucose solutions with known concentrations should be prepared. For instance, five solutions containing 0.10, 0.20, 0.30, 0.40, and 0.50 M of glucose.<br /><br />### Creating a Color Graph: <br />Benedict's reagent should be added to each of these solutions, then heated in a water bath for the same fixed time as your sample (3 minutes in this specific case). The color produced should be matched against a colorimeter, which measures the absorbance of light by solutions.<br /><br />### Plotting a Standard Curve: <br />A machine called a colorimeter can be used to plot the standard curve (absorbance vs. concentration). Each known solution's absorbance value is measured using the colorimeter.<br /><br />## Step3:<br />With the color graph or standard curve plotted from known solutions, you can use these to either use visual comparison (qualitative analysis) or a colorimeter (quantitative analysis) to estimate glucose concentration of your samples/ solutions in question.<br /><br />### Estimation of Glucose concentration: <br />The absorbance of the unknown solutions (experiencing the Benedict's test and heated for 3 minutes) should then be measured using the same colorimeter to determine their concentrations.
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